Microbial control of mosquitoes: Microbes as Bio-larvicides and Fitness indicators of Dengue Vector Mosquitoes

Microbial control of mosquitoes: Microbes as Bio-larvicides and Fitness indicators of Dengue Vector Mosquitoes.

Background Use of microbes associated with mosquito vectors that inhibit pathogen transmission, are a recent advancement in mosquito control programs (Wang & Lorena, 2017). Microbes associated with mosquitoes are important in digestion of blood and development of mosquito eggs (Wang, et. al., 2017). to decrease mosquito susceptibility to pathogens (Barik& Acharya, 2020) larval mortalities developmental delays to reduce overall fitness Recent reports of mosquito microbes that affect mosquito fitness, Surface and environmental Bacteria ( Bacillus , Straphylococcus , Pseudomonas and Aeromonas - Rami, et al., 2018). Intracellular eukaryotes in mosquitoes ( Microsporidia species-Herren, et. al., 2020; Nattoh , et al., 2021) Intracellular bacteria ( Wolbachia -Rainey, et al., 2014)..

2.Identify microbes associated with Ae. albopictus and Ae. aegypti that affect female fitness and vectorial capacity..

Materials and Methods. Materials and Methods. Mosquito sampling and rearing.

Microbes of Aedes Mosquitoes. Morphologies CFUs Size Shape and elevation Transparency/ pigmentation Texture Colony margins Blast results ncbi ID seq label 1 31x10 7 x5 large circular, raised transparent, white spot in middle, circles milky entire Pseudomonas monteilii MN744370.1 686 2 22x10 7 x5 small circular, raised transparent, white spot in middle, circles milky entire Pseudomonas sp MN179818.1 685 3 40x10 7 x5 medium circular, levated transparent, white spot in middle, circles milky entire Enterobacteriaceae bacterium PXS KC410779.1 095 4 38x10 7 x5 tiny circular, raised slightly reddish, transparent milky entire Pseudomonas mosselii strain MT598025.1 096 5 126x10 8 x5 large circular, elevated opaque sticky entire yeast 668 6 200x10 7 x5 medium circular, raised white slimy entire Enterobacter sp. VJ-3 strain KX226332.1 097 7 348x10 7 x5 small circular, raised opaque milky entire Serratia marcescens OL824933.1 670 8 126x10 7 x5 small Circular, raised yellowish and white slimy entire Enterobacter hormaechei CP042566.1 671 9 605x10 7 x5 small circular, flat opaque milky entire Serratia nematodiphila OQ561198.1 672 10 35x10 7 x5 medium circular, raised slightly redish milky entire Citrobacter freundii MT075839.1 673.

unf /SOleWOJ JO qwßOl ßu!MOJOJ oßeuonv. Association between Physicochemical properties of breeding water and body size index of female mosquitoes (wing length).

Effects of microbes on growth and Development of dengue mosquito larvae.

Effect of d ifferent combinations of microbes on the development of dengue mosquitoes.

Key Achievements. Identification and characterization of twenty mosquito microbes.