OUCHTERLONY IMMUNODIFFUSION. Double Immunodiffusion. 

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Double Diffusion. Agar plate. Icon Description automatically generated. 

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OUCHTERLONY DOUBLE DIFFUSION-. Used for comparison of antigens.. 

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Ag1. Ag2. Ab. Icon Description automatically generated. 

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Precipitin bonds formed in this reaction can be   classified into three types, which indicate whether or not they share epitopes.      1) Pattern of Identity           2)pattern of Non-Identity          3)pattern of partial Identity

Case1: Given two antigens are Identical. Ag1. Ag2. 

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Precipitation line will fuse and result in an smooth line of precipitation band. Two precipitation line will overlap and Ag concentration will increase resulting in fusion of precipitation lines.

Ag shares all the epitopes and hence they are identical

Case2: Give two antigens are Non- Identical. Ag1. 

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Case2: Give two antigens are Non- Identical

Cross  each other without any Interaction.

Antigens have no common epitopes and hence they are non-identical

Case3:Given two antigens are partially identical. 

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Case3:Given two antigens are partially identical

Incomplete cross  or an  arc with a spur.

Antigens  share one or more epitopes ad they are partially identical.

Ouchterlony Double Diffusion:. Precipitation Pattern. 

OUCHTERLONY IMMUNODIFFUSION

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