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Recombinant DNA Technology. Let’s “play” with DNA.
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What nature does blindly, slowly and ruthlessly, man may do providently, quickly and kindly. Sir Francis Galton In Nature (2 Feb 1911), 85, 444. Let's say intelligence is your ability to compose poetry, symphonies, do art, math and science. Chimps can't do any of that, yet we share 99 percent DNA. Everything that we are, that distinguishes us from chimps, emerges from that one-percent difference. Neil deGrasse Tyson.
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Recombinant DNA Technology- An Introduction. Recombinant DNA technology is a set of molecular techniques for locating, isolating, altering, and studying DNA segments. The term recombinant is used because frequently the goal is to combine DNA from two distinct sources. Genetic engineering.
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Previously, information about the structure and organization of genes was gained by examining their phenotypic effects, but the new technology makes it possible to read the nucleotide sequences themselves..
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Natural Selection vs and .tretr•hiog Artificial Selection Corn and Its Ancestors stretching higinal neck up tree Driven by inner • nger Teosinte Intermediates Modern corn.
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Series of process – Steps in rDNA. Step 1: The DNA fragment containing the gene sequence to be cloned (also known as insert) is isolated. Step 2: Cutting DNA (Restriction enzymes) Step 3: Joining DNA into vector (Ligases).
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Series of process. Step 4: Transfer of the rDNA molecules into an appropriate host cell. Step 5: Selection of the host cells carrying the rDNA molecule using a marker. Step 6: Replication of the cells carrying rDNA molecules to get a genetically identical cells or clone..
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[Virtual Presenter] Figure 20.2 A preview of gene cloning and some uses of cloned genes..
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Applications. Following are the main areas in which genetic manipulation can be used: Basic research on gene structure and function Production of useful proteins by novel methods Generation of transgenic plants and animals Medical diagnosis and treatment Genome analysis by DNA sequencing.