Plant Genetics CPSC 352
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Plant Genetics CPSC 352. DNA Structure, Replication, and Repair Dr. Anthony J. Studer.
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DNA Structure, Replication, and Repair. Lecture Byte Objectives: DNA is Genetic Material DNA Structure DNA Organization.
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DNA as genetic Material. Erwin Chargaff (1940s) Different species have different proportions of nucleotides Always 1:1 ratios of A:T and G:C Avery, MacLeod, and McCarty (1944) DNA is the unit of heredity not protein.
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Centrifuge IIIS cells spun to bottom of tube Heat-kill Treat with ribonuclease Homogenize cells Recover IIIS filtrate Extract carbohydrates, lipids, and proteins IIIS cells in liquid culture medium Treat with deoxyribonuclease IIR cells DNase-treated IIIS filtrate Test for Transformation IIR cells RNase-treated IIIS filtrate Treat with protease IIR cells Protease-treated IIIS filtrate No transformation occurs only IIR cells Conclusion: Active factor is DNA IIIS cells Transformation occurs IIR cells IIIS cells Conclusion: Active factor is not RNA Transformation occurs IIR cells IIIS cells Conclusion: Active factor is not protein IIR cells IIIS filtrate Transformation occurs IIR cells IIIS cells Control: IIIS contains active factor.
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DNA as genetic Material. Erwin Chargaff (1940s) Different species have different proportions of nucleotides Always 1:1 ratios of A:T and G:C Avery, MacLeod, and McCarty (1944) DNA is the unit of heredity not protein Hershey Chase (1952) Additional Evidence.
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Why Label with 32 P and 35 S?. Figure_09_05_L.
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DNA Structure. DNA (Deoxyribonucleic Acid) Nitrogenous base Sugar (deoxyribose) Phosphate.
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Nucleotides. (a) Pyrimidine ring Purine ring (b) HOCH2 0 OH Ribose NH2 Cytosine Guanine HOCH 2 OH 2-Deo Uracil NH2 OH Thymine NH2 Adenine OH xyribose.
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Rosalind Franklin and Maurice Wilkins X-ray diffraction patterns.
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Essentials of Genetics 9 th Edition, Pearson. The important features of the Watson and Crick DNA structure (1953) Double helix. The strands are antiparallel..
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(b). Essentials of Genetics 9 th Edition, Pearson.
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The important features of the Watson and Crick DNA structure (1953) Double helix. The strands are antiparallel. Purines must pair with pyrimidines. Adenine must pair with thymine and guanine with cytosine. The strands are joined by hydrogen bonds Nobel Prize Awarded in 1962.
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Octamer: H2a, H2b, H3 and H4. DNA Organization. Nucleosome core 1400 nm HI histone (b) Solenoid (30-nm diameter) Histones DNA (2-nm diameter) (d) Metaphase chromosome Chromatid (700-nm diameter) (c) Chromatin fiber (300-nm diameter) Looped domains Spacer DNA plus HI histone Histone octamer plus 147 base pairs of DNA 4 (a) Nucleosomes (6-nm x 1 1 -nm flat disc).
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DNA Organization. Essentials of Genetics 9 th Edition, Pearson.
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DNA Structure, Replication, and Repair. Lecture Byte Objectives: DNA is Genetic Material DNA Structure DNA Organization.