Kamlesh Ph.D. Synopsis

[Virtual Presenter] The School of Biotechnology at Shoolini University of Biotechnology and Management Sciences has developed a novel bacterial-algal consortium for bioremediation with the aim of degrading, decolorizing, and detoxifying effluent from the textile industry. This research is led by research scholar, Mr. Kamlesh, who holds a B.Sc. and M.Sc. degree, and is supervised by Dr. Gaurav Saxena, who holds a M.Sc., Ph.D., and NET. With this research, we are trying to create a next-generation bioremediation technology for the paper industry..

SUPERVISORY COMMITTE.

[Audio] The slide presents a research project on developing a bacterial-algal consortium for the textile industry to tackle the environmental pollution created by its effluent. Background problem, research gap, novelty statement, research hypothesis, research questions, rationale, objectives, proposed research methodology and timeline have been identified. Expected outcomes, significance, and references have also been outlined. It likely will make a significant contribution to the biomedical sciences domain and by extension to the environment. Bacteria and algae might be used together to degrade, decolorize and detoxify effluent from the textile industry, helping to promote a safer environment..

[Audio] The Indian paper industry manufactures printing and writing, newsprint, paperboard, and other paper products and is the 15th largest manufacturer of pulp and paper in the world and the 14th largest exporter of pulp and paper in the world, accounting for 5% of the world's production of paper and contributing 6.9% to India's GDP. In 2023, Foreign Direct Investment (FDI) was $71.2 billion and increasing significantly, with major contributors being China, the US, Japan, Germany, and Korea. To help reduce the environmental impact of this industry, Shoolini University of Biotechnology and Management Sciences School of Biotechnology has recently developed a novel bacterial-algal consortium that can decolorize, detoxify, and degrade their effluent - contributing towards a safer and healthier environment..

[Audio] Paper industry is a key contributor to the economy of many developing countries, including India. However, it is facing serious challenges due to its associated environmental pollution. Effluent generated by paper manufacturing is highly toxic and can cause soil and water pollution, resulting in toxicity to living beings. Thus, it is essential to treat and detoxify this effluent using an environmentally friendly and sustainable technology, to achieve sustainable development with minimal environmental impact. Shoolini University of Biotechnology and Management Sciences, School of Biotechnology, has taken a positive step forward in this direction by developing a novel bacterial-algal consortium to degrade, decolorize and detoxify effluent from the textile industry..

[Audio] Textile industry has a wide range of environmental repercussions, from carcinogenic and mutagenic effects to endocrine disruption and neurotoxicity, or the production of hazardous byproducts due to the Maillard reaction and eutrophication. These byproducts can lead to soil pollution, water pollution, and bioaccumulation of contaminants in the food chain. In order to combat these adverse consequences, the School of Biotechnology at Shoolini University of Biotechnology and Management Sciences has developed a bacterial-algal consortium that has the capability to degrade, decolorize, and detoxify textile industry effluent, thereby safeguarding the environment..

[Audio] Today I will be discussing an important research project which has been undertaken by the School of Biotechnology at Shoolini University of Biotechnology and Management Sciences. This project aims to develop a novel bacterial-algal consortium to degrade, decolorize and detoxify effluent from the textile industry for environmental safety. To this end, this project will focus on the occurrence and fate of emerging contaminants in pharmaceutical wastewater treatment, looking specifically at the nature and characteristics of these emerging contaminants, as well as their toxicity. Moreover, the project also seeks to standardize methodologies for the detection of emerging contaminants in pharmaceutical wastewater, as well as to investigate microalgal strains that may be suitable for the removal of such contaminants from pharmaceutical wastewater. Unfortunately, traditional activated-sludge treatment process is insufficient, and physicochemical treatment technologies may be more effective but can lead to secondary pollution and high operational and treatment costs. Emerging effluent treatments, such as membrane bioreactors, membrane filtration technologies, and advanced oxidation technologies are also energy-intensive and uneconomical. Finally, combined treatment technologies which employ both biological and physicochemical treatments require major changes to the existing wastewater treatment plans..

Table 1: Discharge standards for PME..

[Audio] We are proposing a novel strategy utilizing a bacterial-microalgal consortium for the treatment and detoxification of effluent from the textile industry. This innovative approach aims to address the issues associated with the traditional methods of bioremediation such as high operational costs, the need for a large space, generation of a high amount of sludge and difficulty in biomass harvesting and valorization. Through this research, we hope to develop an effective and cost-efficient solution that will meet the requirement of environmental and public safety..

[Audio] "One of the most efficient and sustainable methods for polluting wastewater treatment is by using microbial consortia. Our School of Biotechnology has developed a novel bacterial-algal consortium to do just that. The advantages are plentiful! It provides for efficient pollutant remediation, higher biomass production, improved biomass harvesting, enhanced resistance to invasive species and a decrease in aeration requirements. Moreover, it also improves the quality of treated wastewater, increases the ability for varied treatment conditions and facilitates the production of multiproducts via biomass valorization. This novel consortium is a great step forward in protecting our environment by creating a safe and sustainable system of treatment for wastewater from the textile industry..

[Audio] Our School of Biotechnology at Shoolini University of Biotechnology and Management Sciences has been researching the use of bacterial-algal consortiums to degrade, decolorize, and detoxify effluent from the textile industry for environmental safety. We have identified several research questions, such as how living organisms respond to this effluent, how and why bacteria and microalgae form consortiums in nature, and how microalgal-bacterial consortiums for remediation can be effectively developed. We are also studying which mechanisms bacteria and microalgae employ to survive in consortia and remediate pollutants and effluent from the paper industry, as well as which factors trigger the bioremediation of paper industry effluent using microalgal-bacterial consortium. Our research is important for understanding how to use natural resources to improve water quality and protect the environment..

[Audio] This presentation is discussing a novel bacterial-algal consortium created by the School of Biotechnology at Shoolini University. This consortium is able to degrade, decolorize, and detoxify effluent from the textile industry. This is an important advancement when it comes to environmental safety, as effluent from the textile industry needs to be carefully treated and detoxified before it is released into the environment. This study will explore new ways to improve existing effluent treatment plants and integrate them with the microbial-algal consortium. This is an urgent need, as it will help to conserve aquatic resources and ensure environmental safety..

[Audio] Shoolini University of Biotechnology and Management Sciences has taken a groundbreaking step in resolving effluent issues from the textile industry. The School of Biotechnology has developed a novel bacterial-algal consortium that can effectively degrade, decolorize and detoxify the effluent, greatly increasing environmental safety. The consortium is based on a mutualistic relationship between microalgae and bacteria, allowing both species to benefit from the exchange of nutrients between them. The microalgae supply oxygen and dissolved organic matter to aid bacterial activity, as well as providing a secondary habitat. In return, the bacteria offer carbon dioxide, fixed nitrogen, vitamin B, and siderophores to improve microalgal growth and flocculation. Furthermore, the bacteria and microalgae interact and exchange metabolites via sensing and reacting to chemical signal molecules, enabling both species to work in sync to optimize their functioning. This advancement is a significant step in reaching stricter standards in wastewater treatment and ensuring environmental safety..

[Audio] Shoolini University of Biotechnology and Management Sciences' School of Biotechnology has developed a novel bacterial-algal consortium to tackle environmental risks from the textile industry. This consortium can effectively degrade, decolourise and detoxify the wastewater from the textile sector. It is composed of bacteria, microalgae and a phycosphere, while the system's pH and temperature have been precisely monitored. The consortium also releases beneficial metabolites such as antibiotics and algicidal substances, which restrict the production of toxins like quorum sensing, microcystin and autoinducer. Furthermore, it produces organic carbon and oxygen, carbon dioxide and bicarbonate, exopolysaccharide and other vital inorganic substances, vitamins and minerals. This consortium reduces the amount of biomass and cell mass, as well as organic carbon and nitrous nutrients. It is an excellent example of how biotechnological research can be applied to an environmental issue..

OBJECTIVES.

[Audio] I am going to present the research conducted by the School of Biotechnology at Shoolini University of Biotechnology and Management Sciences. The research is focused on bioremediation of paper industry effluent using a newly developed bacterial-microalgal consortium. In order to create the consortium, isolation, purification, screening and characterization of bacterial and microalgal strains were done which are able to degrade and detoxify the organic pollutants contained in the effluent. A study was done to examine the physicochemical parameters, ROPs, and their metabolic products present in the paper industry effluent before and after treatment with the newly developed bacterial-microalgal consortium. This research gives a potential, successful approach for bioremediation and environmental protection..

[Audio] Our research looks into the effects of industrial waste on the environment by collecting and analyzing paper industry effluent, paper sludge, and water samples from two sites in India. These samples will be taken from the outlet of Star Paper Mills Limited in Saharanpur, Uttar Pradesh, and from Renuka Lake in Sirmaur district of Himachal Pradesh. To analyze these samples, we will use multiple lab tests and experiments, including physicochemical analysis, bacterial and microalgal strain isolation, bioremediation studies, and ecotoxicity assessment. The goal of this research is to create a novel bacterial-algal consortium that can break down, decolorize, and detoxify effluent from the textile industry to ensure environmental safety..

[Audio] Our research team at Shoolini University of Biotechnology and Management Sciences has successfully developed a novel bacterial-algal consortium which can thoroughly degrade, decolorize and detoxify effluent from the textile industry. To identify optimal bacterial strains for the degradation of ROPs, our team applied a series of serial dilution and streaking methods, before going on to screen the isolates for the presence of catabolic ligninolytic enzymes. To authenticate the potential bacterial strains, they utilized a range of morphological and biochemical techniques, in addition to 16s rRNA gene sequencing and BLAST analysis. The results of this work demonstrate that the consortium is able to efficiently degrade and detoxify ROPs present in paper industry effluent..

[Audio] The School of Biotechnology at Shoolini University of Biotechnology and Management Sciences has created a unique bacterial-algal collaboration to help secure environmental safety. This consortium was developed to degrade, decolorize, and detoxify liquid waste from the textile industry. To fulfill this objective, the School selected a microalgal strain using sequential dilution, purification, and sub-culturing techniques. The selection was based on the PIE decolorization assay and growth potential. The strain's culture conditions were kept at 25°C with a 12/12h light/dark cycle and cool-white fluorescent light illumination at 4000-5000 lux. Morphological and biochemical characterization was executed, followed by molecular characterization of the 18S rRNA gene sequencing, and identity confirmation using BLAST analysis. Finally, an accession number was obtained via GeneBank. With such efforts, the School of Biotechnology has developed an efficient bacterial-algal consortium for environmental safety..

[Audio] Without greetings and without beginning with "Today": The School of Biotechnology at Shoolini University of Biotechnology and Management Sciences has developed a novel bacterial-algal consortium to degrade, decolorize, and detoxify effluent from the textile industry. Potential remediating bacterial strains are co-inoculated with potential microalgae strains in different combinations and then subcultured into the fresh BG11+PME for up to 35 days for acclimatization. This allows for a stable growth of the consortium which can then be used in bioremediation of paper industry effluent. We believe this consortium will be effective in degrading and detoxifying ROPs in PIE and can be used with success for environmental safety. Thank you for listening to this presentation..