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Safinaz  zaki   shabaan Clinical pathology consultant Clinical hematology specialist Harvard alumni association Egyptian fellowship trainer

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2 - Stain  to see morphology:.  Commenly  used stains in blood film  Leishman  and  Gimsa  stains. Leishman  stain:  Methyline  blue  (basic dye) for stain acidic component and give blue color                                  eosin  (acidic dye) for stain basic component and give red color. Giemsa   stain:  Methyline   blue and Azure  (basic dye) for stain acidic component and give blue  color  eosin  (acidic dye) for stain basic component and give red color . RED  COLOR CELLS: RBCs and some granules. BLUE  COLOR CELLS: nucleus and some granules.

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3- Cells  to see morphology:   1-type of cells ; RBCs, WBCs, Platelets. 2- Component of each cell:  nucleus and cytoplasm

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WBCs:     - granulocytic  cells (cytoplasm contain granules ) :  -neutrophil -eosinophil  -Basophil - agranuolocytic  cells (cytoplasm don’t contain granules ) :  -lymphocyte  -  monocyte

Neutrophil
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(a) Granulocytes
Basophil
Monocyte
Lymphocyte

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Eosinophil
• Diameter: 13.14 micms
• Cytoplasm : full of orange•
red coam granules
u Nucleus:
• blue like a pair of
glass

reasonable.
A polyxnorphonuclear white blood cel

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Mark the best field in peripheral blood film to see best morphology of blood cells Mark the nucleus of RBC

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CBC morphology

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