Applied Microbiology Bioc 429

[Audio] This course provides a comprehensive coverage of the practical use of microorganisms in industrial and environmental applications. The types of bioreactors are explained in the light of the biochemistry of microorganisms and their applications. Moreover, common microbial growth models are explained and used to study growth and production kinetics in various bioprocesses..

Outline. Chapter 1 : Introduction Microbes and man Microbiology vs. Chemistry Microbial growth Chapter 2 : Bioreactors A bioreactor Choice of the bioreactor type Chapter 3 : Industrial applications Biomass production Biosynthesis of primary metabolites Bioconversion to secondary metabolites.

Chapter 1 Introduction part A. 3.

[Audio] Fermentation products Beer: using straws, spent barley grains were not separated Rules: people who consumed beer in excess were condemned Hymn to ninkasi was left outside to allow the growth of.

[Audio] Kombucha: fermented tea, functional food in far east in -1000 BC.

[Audio] Attenuated bacteria ( Dutchland) Pasteurization: Prevention of beverage deterioration Bacillus : bread deterioration – becomes liquid, pure cultures dominate and prevent Bacillus proliferation The activated sludge process is a type of wastewater treatment process for treating sewage or industrial wastewaters using aeration and a biological floc composed of bacteria and protozoa.

[Audio] ( Russia) Russian steppes Microbial therapy suppressing putrefactive colonic bacteria consisted of daily doses of probiotics in the form of "soured milk (i.e., yogurt) Metchnikoff in 1907 hypothesized that replacing or diminishing the number of 'putrefactive' bacteria in the gut with lactic acid bacteria could normalize bowel health and prolong life. Over a century ago, Elie Metchnikoff theorized that health could be enhanced and senility delayed by manipulating the intestinal microbiome with host-friendly bacteria found in yogurt. His theory flourished for a time, then drifted to the fringe of medical practice before re-emerging in the mid- 1990s Nobel Laureate Elie Metchnikoff discussed the benefits associated with Kefir in his Theory of Longevity. From that point on, scientists have continually reaffirmed the medicinal benefits of fermentation due to the "healthy bacteria" found in the foods..

[Audio] Bioreactor kinetic models: same period as enzymatic kinetic model of Michaelis and Menten, based on them.

Bioproduction Chemical production Impure raw materials, simple upstream process Purified raw materials Final p roduct obtained in few steps One molecular transformation at each step Complicated downstream process Concentrated products Energetically sustainable (low T) Energetically greedy (higher T) Slow Fast Control at several levels in the operational system Simple control of physical parameters Biodegradable waste (large amounts) Recalcitrant waste (low amounts).