Name: PowerPoint Presentation
Uploaded: 2022-08-22
Duration: 1 min 41 s
Description: Hardening Techniques. By Bhavadharani V 2018030015.

Hardening Techniques. By Bhavadharani V 2018030015. 

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Hardening:. Hardening is the incubation of plants in plastic pots for 6 to 8 weeks in a greenhouse. It is the acclimatisation of tissue culture plants slowly before growing in the field.. 

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Hardening is the incubation of plants in plastic pots for 6 to 8 weeks in a greenhouse.  It is the acclimatisation of tissue culture plants slowly before growing in the field.

Selection and preparation of Mother plants Culture initiation Multiplication and Elongation Rooting of shoots Transfer of plants to soil

Three Types of Hardening Procedures:. 1.In-vitro hardening. 

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What is the need for hardening ?. Photo autotrophic. 

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Plantlets subjected to  specific culture regimes  aimed at making them capable of surviving the harsher ex- vitro environment Specific culture regimes Modified nutrient media & culture conditions Chemical additives in culture media Coculture with micro organisms Photoautotrophic micro propagation

Exposed to full sunlight. Ex vitro hardening. Rooted shoots are removed. 

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Transplanted into plastic cups with sterile media

For 10 to 15 days, High RH 90% maintained (Plastic bags or sheets) with low light intensity

Primary Hardening Greenhouse UV stabilized polysheet Water repellent benches Four way misting system Thermometer Hygrometer Lux meter Secondary Hardening Shade house 50% light cut out black shadenet Drip irrigation Fertigation Micro sprinklers. 

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Primary Hardening Greenhouse UV stabilized polysheet Water repellent benches Four way misting system Thermometer Hygrometer Lux meter Secondary Hardening Shade house 50% light cut out black shadenet Drip irrigation Fertigation Micro sprinklers

Plants ready with 3-5 well developed leaves with good mass of fibrous roots

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