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Gingoog-Claveria-Villanueva Road 3.m4a. UNIVERSITY OF SCIENCE AND TECHNOLOGY OF SOUTHERN PHILIPINES Size-Exclusion Chromatography SEPARATION TECHNIQUE.

Gingoog-Claveria-Villanueva Road 4.m4a. Description of the Technique.

New Visayas 5.m4a. Ize-ExcIusion Chromatograph (SEC) Size exclusion chromatography (SEC) separates molecules based on their size by filtration through a gel. The gel consists of spherical beads containing pores of a specific size distribution. Separation occurs when molecules of different sizes are included or excluded from the pores within the matrix. Small molecules diffuse into the pores and their flow through the column is retarded according to their size, while large molecules do not enter the pores and are eluted in the column's void volume. Consequently, molecules separate based on their size as they through the column and are eluted in order of decreasing lecular weight..

30 Dec, 9.21 am​.aac. Schematic Diagram Injector Solvent delivery system Solvent Column Detector (s) Waste Recorder/ data system.

RPReplay_Final1640833955.mp4. Schematic Diagram SIZE - EXCLUSION C H RO MA TO MATIC DIA C, RAM SAMPE W kræ IN J SOLVENT sup PLY CDN,-TANT gmys or CA-ur.dNC THE.

Gingoog-Claveria-Villanueva Road 4.m4a. Applications Operating conditions and gel selection depend on the application and the desired resolution. Two common types of separations performed by SEC are fractionation and desalting (or buffer exchange.).