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Standard Chartered-Shroff ’ s  Eye Care Education Academy

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Epithelial monolayer Thick basement membrane Avascular, hypocellular stroma Compact layer Fibroblastic layer Spongy layer

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PROMOTES EPITHELIALISATION Promotes  epithelial cell  migration, adhesion  and  differentiation ANTI -INFLAMMATORY Inhibition  of the expression of  proinflammatory cytokines by HC-HA/PTX3 complex  such as interleukin (IL)-1a, IL-2, IL-8, IL- 10, IFN-c, bFGF, TNF- β   and PDGF from the damaged ocular surface Fas receptor mediated  apoptosis of inflammatory cells ANTI-SCARRING Suppression of TGF b signaling Promotes  epithelial  progenitor cells growth  and  clonigenicity Prevents  epithelial cell  apoptosis

Jirsova K, Jones GL. Amniotic membrane in ophthalmology: properties, preparation, storage and indications for grafting—a review. Cell and tissue banking. 2017 Jun 1;18(2):193-204.

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ANTI-ANGIOGENIC Expression of  thrombospondin-1, endostatin  and  tissue inhibitors of metalloproteases  (TIMP-1, 2, 3 and 4) ANTI-MICROBIAL Presence of  bactricidin, beta-lysin, lysozyme, transferrin and 7-S immunoglobulins IMMUNOMODULATORY  EFFECT Limited expression  of  HLA class Ia  (HLA-A, B, C, DR) and  class Ib  (HLA-E, G) antigens by AM epithelial and mesenchymal cells

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CONJUNCTIVAL SURFACE RECONSTRUCTION  Pterygium surgery  Chemical burns  Cicatrizing conjunctivitis  Ocular surface squamous neoplasia (OSSN)  Symblepharon release  Fornix formation  Conjunctivochalasis

CORNEAL SURFACE RECONSTRUCTION  PEDs, Shield ulcer  Non-healing stromal ulcers  Partial LSCD  Total LSCD  Limbal dermoid Bullous keratopathy Band keratopathy  Scleral melt

Sangwan VS, Burman S, Tejwani S, Mahesh SP, Murthy R. Amniotic membrane transplantation: a review of current indications in the management of ophthalmic disorders. Indian journal of ophthalmology. 2007 Jul 1;55(4):251.

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SURGEONS : DR. VIRENDER S.SANGWAN, DR. AASTHA SINGH, DR. NIDHI GUPTA CONCEPT & SCRIPT : DR. AASTHA SINGH RESEARCH & PRESENTATION : DR. GARIMA CHAUDHARY

AMNIOTIC MEMBRANE GRAFTING

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