Now we understand the importance of analysis of mercury, so let's check out the method.

Now we understand the importance of analysis of mercury, so let’s check out the method..

A rapid spectrophotometric method for the determination of mercury in fish samples using diphenylthiocarbazone.

Procedure:. A series of standard solution of a neutral aqueous solution containing 1–250 µg of mercury(II) in a 10-ml volumetric flask was mixed with 0.8 ml of 1.45×10−3 M of the diphenylthiocarbazone reagent solution followed by the addition 0.1 ml of 4.5 M sulphuric acid (0.45 M)..

. 10–20 g of fish sample was taken in a 100 ml micro-Kjeldahl flask with a B24 socket attached to a standard double surface reflux condenser..

According to the EPA, the highest allowable average mercury concentration in fish per serving when eating 1 serving per week = 0.46 µg/g..